Guppy Kits

Observing how predators preferentially chose
from certain environments
(guess which ones?)

I have been involved with a major outreach project to create self-guided kits for teaching evolution in K-12 classrooms. This project is a collaboration between me (as part of my broadening participation efforts on my NSF grant), Dale Broder (a senior graduate student whose work we are building off of), Dr. Cameron Ghalambor (who has helped Dale implement a similar program with local 7th grade classrooms), Dr. Lisa Angeloni (who has helped with several other similar outreach projects), and the CSU College of Natural Sciences Education and Outreach Center (yes, we have one of those!). The goal for these kits is three-fold: 1) to be able to expand our program beyond what we can reach ourselves (this is where the EOC comes in!), 2) to increase classroom engagement by using live animals, and 3) to use an authentic science approach to increase scientific self-efficacy (help students realize they CAN actually do science) and learn about evolution the same way scientists have done. We are particularly interested in implementing this program in schools that serve low-income and underrepresented students. To address potential psychological barriers these students may have (which they may not even realize), we have made a significant effort to include scientists representing several aspects of diversity, both in an introductory video the students will watch prior to starting the activity, as well as with an interview included in the final pages of the instruction booklet. These kits will be directed toward middle and high school students initially, but could also be adapted for other age or ability levels as well.

Follow along here as I post updates on how these kits are coming along!

UPDATE 9/17/15
Well, as far as an update goes, this is a little late as we have made significant progress on the kits since we first met with the EOC back in May. We have also submitted an abstract for a poster at the upcoming Society for Integrative and Comparative Biology meeting in Portland, OR (see my previous post).

The activities for the kits include:
1) Observe the variation in physical differences between males from three populations of guppies (pet store, wild low predation, wild high predation).
2) Understand how color patterns are passed on between parents and offspring using punnett squares.
3) Observe guppy predator avoidance behaviors and quantify survival when exposed to predators (this will be done using pre-recorded high-speed videos).
4) A thought exercise to address how color patterns change over time due to various types of selection.

In the last few weeks, Dale and I have figured out how to assemble small acrylic tanks that don't leak (not a minor task). These will be used as small observation tanks.

Dale gluing a tank

The finished product!
I have also been filming Catalina, one of our pike cichlid predators, capturing guppies. She has a choice between representative males from each of the populations. I used a go-pro to film at 120 fps but the video below is from my Nikon DSLR (not high speed).

We have also been working hard on assembling the introductory video, with the help of a local videographer. This included recording an interview with each of our scientists using some local news crew equipment that Dale borrowed.

Interviewing Cameron in the breeding lab

If that wasn't enough, we recruited an undergrad, Ty Fiero, to help with 3D printing some guppy stencils, so the students can draw a fish that they can then add fins to and color in.

Yes, those are glow-in-the-dark!
I drew them myself!

We have also been working closely with Andrew Warnock, from the EOC, to compile the instruction booklet that the students will use. This included coming up with a name for our kits.

It's coming together! Our goal is to be finished in time for a teacher workshop in October.

UPDATE 09/18/15

We now have predator silhouettes for students to test how each guppy might respond to predators. They will then make predictions about what they think will happen with real predators. I made a range of sizes to figure out which one works best.

UPDATE 10/29/15

So we didn't make our October deadline...

We ran into some hurdles with IACUC approval as well as trying to figure out better options for the observation tanks. We've decided to change the teacher workshop to January, which will actually work out better for when they will teach evolution in their classes. Plus it gives us a little more time to make everything just right.

Today we were finally approved to move fish to the Education and Outreach Center!  We had to set up the tanks first without fish so they could inspect the room. Now that we're approved, I added some living things today - some nerite snails and some aquatic plants, Vallisneria and Ludwigia. Next comes guppies...

20g tank for petstore guppies
10g tanks for wild guppies

UPDATE 12/10/15

We're making the final push for our guppy kits! Dale and I have put a lot of work into the instruction booklet, and just wrote the final pages outlining the 4 components of Evolution the students worked through (Variation, Inheritance, Selection, and Time). We also provided some FAQs in case students have more questions about the projects they do.

We also finalized our introductory video that the students will watch before they start. This will be used to introduce the guppy system and let them hear from real scientists.

We encountered a hurdle recently regarding the observation tanks in the kits - do we ask the students to observe one guppy at a time and rotate, or do we ask the students to observe multiple guppies at a time, and if so, how do we facilitate that? Since variation is an integral concept for students to understand, we decided we wanted the later option, so then the question became do we put multiple fish in one tank or do we include multiple tanks. One of our collaborators had the idea to make nesting tanks, so we gave that a shot! They turned out well, so I think this is what we will go with.

Two nested observation tanks
 We also encountered a problem with the most recent set of 3D printed stencils - that a pencil wouldn't fit in the lines. So we asked Ty to print it one more time, hopefully for good this time. As of 10am this is what they looked like:

3D printing guppy stencils (Photo by Ty Fiero)

Here are the completed stencils, and what the traces look like:

The guppy stencil finished product!
This is going to be AWESOME!

The last piece of this puzzle that I have been working on is the poster that I will present at SICB this January. Dale and I will be co-presenting our poster (abstract #P1-7) on
Monday Jan. 4 from 3:30pm-5:30pm in Exhibit Hall A

Also, if you're interested in hearing about the work that led up to the projects we included in the kits, Dale is presenting a talk (abstract #110-3) on
Thursday Jan. 7 at 9am in room B115 

UPDATE 12/18/15
The first full draft of the instruction booklets have been printed!

Andrew did the illustrations!
We also now have an "official" kit pelican case! Blue is the perfect color for something with fish!

It looks like a kit now!

Everything fits inside!
AND...the poster is finished! SICB 2016: ready or not, here we come!

Well I guess you'll just have to come see our poster!

UPDATE 1/12/16
The conference went really well! There was more interest in our kits than I think I have had in all my previous SICB presentations combined! It's so great to see that people like what we're doing and think our work will be useful, not only for elementary and high schools, but also intro-level college labs. The two questions that literally EVERYONE asked:

1) Does it work?
    For that answer (essentially, yes), you'll have to wait until Dale's paper is published.

2) When/how can I get one?
    Great question, we still have to classroom-test the kits and make any necessary changes. We're targeting about 1.5 hr to complete the activities, but if that doesn't happen we'll have some work to do. Once we are satisfied with the contents of the kits, we will make all materials available via the CSU Education and Outreach Center. Once that happens, I will provide a link on this page.

If you missed our poster, or just loved it so much you want to see it again, here it is:

SICB 2016 Portland poster
Please spread the word about our kits, and check back to see how we are progressing! Thanks again for all the support and interest!

UPDATE 2/29/16 (happy leap day!)
The Education and Outreach Center has printed flyers to advertise the kits! Check out their website to learn more about checking out the kits once they are finished. We have three dates planned in March and April for hosting students here at CSU to test out the kits! Hopefully soon after that we will start allowing teachers to check out the kits and guppies for use in their classrooms.

We also spent our monday lab meeting today building the nesting tanks that fit inside the pelican cases. These will be observation tanks that will house a guppy from either the high or low predation environments during the activities. Thanks to our labmates for all their help and to Rachel Bockrath for taking photos!

Chris Kopack, Dale Broder, John Kronenberger,
and Michelle Moyer building acrylic tanks

Lisa Angeloni helping me glue acrylic

30 nested tanks, finished!

UPDATE 3/25/16

Ready or not (we were definitely ready), today was the day! We had our first run with 24 real, live students from Ms. Jordan's 7th grade class at Wellington Middle School. STEM fridays are an event hosted by the Education and Outreach Center (more info here) where local teachers are invited to bring up to 30 students to CSU to participate in their chosen kit activity. It's a chance for students to come to CSU and see what it's like, as well as an opportunity to learn something in the process. I must say, as my first time doing one of these events, and our first time doing it with the guppy kits, it went fantastically! As we expected, there are some changes to be made, but the students were happy, excited, and involved, which is what we were hoping for.

Here are some photos, more are posted elsewhere:

Examining differences between guppies
Making punnet squares
Using a predator silhouette to predict predator
avoidance strategies of each population
Watching predator-prey encounter videos
Discussing which fish is from which population
Final thoughts in science notebooks

Now we are one step closer to being able to share our kit design with everyone!

UPDATE 5/17/17

Local teachers participating in the guppy kits

The Education and Outreach Center, who we have been collaborating with on the guppy kits, sponsored a spring open house to give local educators an opportunity to learn about their STEM kits. This was the perfect opportunity to showcase the guppy kits! We had about 30 participants, including middle and high school students, local teachers, and Dale's mom. See more photos here.

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